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KMID : 0613820060160020259
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Journal of Life Science
2006 Volume.16 No. 2 p.259 ~ p.265
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The Mechanism of Nephrotoxicity Formation of Methotrexate in STZ-Induced Hyperglycemic Rats
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Kim Seok-Hwan
Kim Yeo-Jeong
Lee Joo-Yeon
Kang Hye-Ok
Lee Hang-Woo
Choi Jong-Won
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Abstract
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This study is investigated the effect on mechanism of nephrotoxicity formation of methotrexate(MTX) by examing the induction of hyperglycemic by streptozotocin(STZ). MTX was injected daily at two doses of 3, 6 ㎎/㎏ for 1 week in STZ-induced hyperglycemic rats. Activities of BUN, creatinine and LDH were significantly increased by treatment with MTX in STZ-induced diabetic group when compared to MTX treatment group in normal rats. Renal lipid peroxide content and activities of cytosolic enzyme were significantly increased in the treatment of MTX in diabetic group. The concentration of glutathione and glutathione biosynthesis enzymes were decreased by treatment with MTX in STZ-induced diabetic group. These results suggest that nephrotoxicity of MTX in STZ-induced hyperglycemic rat was caused by activation of renal metabolizing enzymes in cytosol and decrease of glutathione concentration.
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KEYWORD
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Methotrexate, streptozotocin, nephrotoxicity, cytosolic enzyme, glutathione
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